Part:BBa_K4773006:Experience

Applications of BBa_K4773006

Figure 1. Isatin production (A) and measured yields (B) by engineered bacteria in LB medium with or without 1 mM indole addition. Control is the E.coli introduced with the empty vector, ycnE is the engineeredE.coli expressing FMO and ycnE, (+) indicates exogenous addition of 1 mM indole.

We assayed the enzyme activity of the ycnE variant in the same manner as before. After culturing the corresponding bacteria overnight, centrifuge and discard the supernatant, then resuspend in M9 medium containing 1mM indole and culture with shaking. After 24 hours, samples were taken to detect the isatin concentration, thus determining the enzyme activity.

Figure 2. Isatin production of wild type and mutant ycnE. The concentration of isatin was determined after 1mM of exogenous indole was added and cultured at 37°C for 24h.

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